Insertion junction: CLIP2.067103_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.067103
Chromosome:plastome
Location:59725
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802287 ChreCp023,atpE,2716972 ATP synthase epsilon chain; (1 of 1) K02114 - F-type H+-transporting ATPase subunit epsilon (ATPF1E, atpC) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTAGGCTCTTCGCGAGACTATGGTCAATTGCCATATATCGGAATATTCAT
Internal bar code:ACGGCTCTGCTCTAACGACCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:129
LEAP-Seq percent confirming:31.5789
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CAGAAAGGACGCTCTGGTGT
Suggested primer 2:TTATCACCGCAGCTTTGGGT