Insertion junction: CLIP2.067124_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.067124
Chromosome:chromosome 12
Location:3534790
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g496450 RRP4 (1 of 1) K03679 - exosome complex component RRP4 (RRP4, EXOSC2); RNA binding component of exosome complex 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGATGTATTAGAATATGCGGTCTCGTGCCTCCTGACGGACAGAAAGGA
Internal bar code:GACACTGAAGTCTAAGTCGAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:5285
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:119
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:119

Suggested primers for confirmation by PCR

Suggested primer 1:CCTAGGCTAGGTACGTTGCG
Suggested primer 2:CTCATGCGTCAATGCTGGTG