Insertion junction: CLIP2.067124_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.067124
Chromosome:chromosome 12
Location:3534796
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g496450 RRP4 (1 of 1) K03679 - exosome complex component RRP4 (RRP4, EXOSC2); RNA binding component of exosome complex 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGCAGCACACGCTTGCCGCAAGGCCAAGCGCCGGTCATGTCTACCACAC
Internal bar code:GACACTGAAGTCTAAGTCGAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:561
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:CTCATGCGTCAATGCTGGTG
Suggested primer 2:CCTAGGCTAGGTACGTTGCG