Insertion junction: CLIP2.067128_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.067128
Chromosome:chromosome 6
Location:8743134
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g310700 RPL36A,RPL36a,RPL41 Ribosomal protein L36a; (1 of 1) K02929 - large subunit ribosomal protein L44e (RP-L44e, RPL44) 3'UTR
Cre06.g310750 COPG1 (1 of 1) K17267 - coatomer protein complex, subunit gamma (COPG); Gamma subunit of COP-I complex 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTCACGCACACTCCCGACCGCTCCCCTTGATGCGATGGGACATCTGTGG
Internal bar code:CAAGGAGATTAGGTCGCATCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:762
LEAP-Seq percent confirming:27.2727
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:CCCATGAGTCGTGTGTCACA
Suggested primer 2:CATCGTGTATGAGACCGGGG