Insertion junction: CLIP2.067135_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.067135
Chromosome:chromosome 5
Location:1157316
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g248550 (1 of 1) K16276 - zinc finger protein-like protein (K16276, BTS) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTTGTGCGGGTGTCATCTAGAGGTCGATGCCTCGCCCCGCTCATATTTG
Internal bar code:ACGACTAACGACTTTCCATTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2501
LEAP-Seq percent confirming:90.1639
LEAP-Seq n confirming:55
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:61

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGTCATTGCTACGCCTGC
Suggested primer 2:GCTGTGGGATAAACGGAGCT