| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.067144 |
| Chromosome: | chromosome 3 |
| Location: | 5595534 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g185600 | OGD3 | (1 of 3) 2.3.1.61 - Dihydrolipoyllysine-residue succinyltransferase / Succinyl-CoA:dihydrolipoate S-succinyltransferase; Dihydrolipoamide succinyltransferase | 5'UTR |
| Cre03.g185650 | CaM-IP4,FAP251 | (1 of 1) PTHR13720//PTHR13720:SF13 - WD-40 REPEAT PROTEIN // WD REPEAT-CONTAINING PROTEIN 66; Flagellar Associated Protein 251 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTAGTCGATTGACAAGTATAACTTGAACAATTAACTGAGTACTTTGAA |
| Internal bar code: | CCTTGTTACTGAAGAGGATTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 870 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTACGTGGTGCTGTGTGA |
| Suggested primer 2: | ACTACATCGGGCATACGCAG |