Insertion junction: CLIP2.067147_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.067147
Chromosome:chromosome 17
Location:5584811
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g739752 FTSHi1,CTAP1,FTSHI1 (1 of 3) IPR000642//IPR003593//IPR003959//IPR027417 - Peptidase M41 // AAA+ ATPase domain // ATPase, AAA-type, core // P-loop containing nucleoside triphosphate hydrolase; Chloroplast-import FtsH-like ATPase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTCGCAATGGCAATTCGGCACATACACTATAGTTTTCGCGAGTCCGGCT
Internal bar code:TTCGTCAAGATGAGTACTAATA

Confirmation - LEAP-Seq

LEAP-Seq distance:1495
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:43
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCCCTTCACATTTCCCCC
Suggested primer 2:GCGCAACAGTTTGACGTCTT