Insertion junction: CLIP2.067147_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.067147
Chromosome:chromosome 17
Location:5584812
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g739752 FTSHi1,CTAP1,FTSHI1 (1 of 3) IPR000642//IPR003593//IPR003959//IPR027417 - Peptidase M41 // AAA+ ATPase domain // ATPase, AAA-type, core // P-loop containing nucleoside triphosphate hydrolase; Chloroplast-import FtsH-like ATPase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGACACGAGTGCCCCCACCCCCACCCCGCCACCCCGCTTTCCTGACGA
Internal bar code:AATTCGATAGTCTTCATTGAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1124
LEAP-Seq percent confirming:20.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCAACAGTTTGACGTCTT
Suggested primer 2:CTCCCCTTCACATTTCCCCC