| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.067160 |
| Chromosome: | chromosome 17 |
| Location: | 2937246 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g719400 | intron | ||
| lncRNA_TCONS_00243817 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGATTGGTGTAGCTTGCCGTGCCGTGCCGTGCACGTGCGGCCCAGTGC |
| Internal bar code: | TGGGTGTCCTGTGTACAAAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 92 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCGTAACCCCAAATCGTC |
| Suggested primer 2: | TAGCATTACGTCGCTCGGAC |