| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.067195 |
| Chromosome: | chromosome 14 |
| Location: | 1476393 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g617350 | (1 of 7) PF00415//PF13540 - Regulator of chromosome condensation (RCC1) repeat (RCC1) // Regulator of chromosome condensation (RCC1) repeat (RCC1_2) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTTCATGCACACCTATGCACAATGGACCTGGCTCGCATCCCAGCACGA |
| Internal bar code: | TTATATTGTGTGTGCATGTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1162 |
| LEAP-Seq percent confirming: | 70.7317 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGCGTCGTGAACTTTCAT |
| Suggested primer 2: | ATTGGGCAGTCGGACGATTT |