| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.067197 |
| Chromosome: | chromosome 4 |
| Location: | 697616 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217954 | (1 of 1) K05850 - Ca2+ transporting ATPase, plasma membrane (ATP2B) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGGTCGCATCGACAACCGCCACGACAGCGCAACGCCGCAGCCTCCAGC |
| Internal bar code: | CATGTCCGTCTAACAGTTGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1084 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTCCTCAGAAACTGCGGA |
| Suggested primer 2: | GGGGTGCCCTGAGGAAATAC |