| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.067204 |
| Chromosome: | chromosome 12 |
| Location: | 7613558 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g555850 | DESI1,DeSI-1 | DeSI-type SUMO protease; (1 of 2) PTHR12378//PTHR12378:SF7 - UNCHARACTERIZED // DESUMOYLATING ISOPEPTIDASE 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTATGGCCTGCGGTGCTGTGCGTTCACAGCTGCAGCTGTACGTGTGCCA |
| Internal bar code: | ATATGCGTAAGAAGGGGGTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1682 |
| LEAP-Seq percent confirming: | 1.92308 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCAGTGCATTGCCCTGAA |
| Suggested primer 2: | TGTGTACGACCTCACCAACG |