| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.067207 |
| Chromosome: | chromosome 17 |
| Location: | 1352513 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g705843 | Chlamydomonas-specific family W protein; (1 of 6) PF06701 - Mib_herc2 (MIB_HERC2) | 5'UTR_intron | |
| Cre17.g705850 | GGK2,PROB2 | Glutamate 5-kinase; (1 of 3) 2.7.2.11 - Glutamate 5-kinase / Gamma-glutamyl kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAGACAGCCGCGCAACAAAACATACCTTAAGTGAAAAACCTTGAGCGA |
| Internal bar code: | AACGTGCTTTTGATGAAGTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1645 |
| LEAP-Seq percent confirming: | 29.7872 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTCCGTTGCGTCGTCTTA |
| Suggested primer 2: | GTACGGTATGCGGAAGGAGG |