| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.067218 |
| Chromosome: | chromosome 2 |
| Location: | 146930 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g074250 | gene_edge/mRNA_edge/5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTACTAAATATGTTTCTTCATACGCTTCACAGGCAATACCCAGTACTGT |
| Internal bar code: | TTCGGTGCGGACTTAACGTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1272 |
| LEAP-Seq percent confirming: | 68.4211 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCACTGACCCAACGAGAT |
| Suggested primer 2: | TACCCTGAACACGTGCTGTC |