Insertion junction: CLIP2.067234_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.067234
Chromosome:chromosome 2
Location:1731202
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g086150 (1 of 100) IPR001810 - F-box domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGTCACACACTGACACCAACGCACGCATGCACCACACACCACTACTCCA
Internal bar code:TGGGGCTCGAGGTTCAGTTTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2318
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:TGACGTCACTGCTAGACTGC
Suggested primer 2:GCTGCTCACCTGTTGTGTTG