| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.067240 |
| Chromosome: | chromosome 4 |
| Location: | 180203 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g216950 | KAS3 | Putative 3-ketoacyl-ACP-synthase (FAS complex); (1 of 1) 2.3.1.180 - Beta-ketoacyl-[acyl-carrier-protein] synthase III / KASIII | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCACAGGCGGCAGCGGACAGAGCTGCAGAGCGCTGATGACCGGCGAG |
| Internal bar code: | ATATTATCACCACCTAGACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2180 |
| LEAP-Seq percent confirming: | 62.5 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCCGTCCATACCAACAGC |
| Suggested primer 2: | CCCACTGCTCTAACATCGCA |