Insertion junction: CLIP2.067243_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.067243
Chromosome:chromosome 17
Location:6425349
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g744097 GT90F49,GT90-49 GT90 family protein 49; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCTCGCAAGTGTACCATGCGCAACGGTGTGCCGCGCGCACACCCCGCAC
Internal bar code:CACCCCAGATTTCGAGTCAATT

Confirmation - LEAP-Seq

LEAP-Seq distance:1048
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCTAGATTTCTGTGTGCG
Suggested primer 2:CAAACTCTCTGAGCCAGCCA