| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.067245 |
| Chromosome: | chromosome 4 |
| Location: | 377077 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217910 | (1 of 25) PF00581 - Rhodanese-like domain (Rhodanese) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCAGTGTTGGCCGTGTGTCAAGGTGTTCGCAACGACTGAGAGGTGTGA |
| Internal bar code: | GAGTGGAATACTATAAGAGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 305 |
| LEAP-Seq percent confirming: | 28.125 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGTTGCAAGGTCACAACA |
| Suggested primer 2: | TTCTTCTTCAGCTGGCCCAG |