| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.067246 |
| Chromosome: | chromosome 12 |
| Location: | 2848118 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g503250 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCGACATCAGTACCCGTACGGCCCGTACTCATCATCGTAACCCTGCCG |
| Internal bar code: | TGTGAAATCTCGTAGCCAACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 533 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCCCCTTTCCCGGCATAC |
| Suggested primer 2: | GCACATCATCTCGTTGCGTC |