Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | + |
Strain: | CLIP2.067253 |
Chromosome: | chromosome 10 |
Location: | 4153455 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g448850 | FAP135,ODA5AK,CAL5 | (1 of 3) 3.4.22.54 - Calpain-3 / Muscle-specific calcium-activated neutral protease 3; Calpain-Like Flagellar Associated Protein 135 | 3'UTR |
Cre10.g448900 | (1 of 80) IPR003882 - Pistil-specific extensin-like protein | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATGTGCCAGCTCAACTGCTAAACACTCAGCAGCGGGTACCGGTGCGGA |
Internal bar code: | GGCTATTTTGGCGGCTAGGGCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1409 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 19 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTGGAGAATGTGCTCACCGT |
Suggested primer 2: | TAGTGCCTGCCTACCAAAGC |