| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.067261 |
| Chromosome: | chromosome 1 |
| Location: | 6161708 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g043817 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTACTGTACCGACGGTAGCTGGCAGGTTGTGTCGATGTTTTGCTGACGC |
| Internal bar code: | TAACTAGTCGATCCGTTATGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4551 |
| LEAP-Seq percent confirming: | 78.6667 |
| LEAP-Seq n confirming: | 59 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGGAAACAACACCTCGGA |
| Suggested primer 2: | TCGTGGGTGTACTTCTTGCC |