| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.067270 |
| Chromosome: | chromosome 11 |
| Location: | 3627486 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g478184 | (1 of 1) 3.2.1.25 - Beta-mannosidase / Mannase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAAGCTGCAGACCAGCCTACCCCAAATCAATACAGTGTGCATAGCTCGA |
| Internal bar code: | TTTAGCACGGATTCGTCCATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1211 |
| LEAP-Seq percent confirming: | 82.8571 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACTCGGAGATGAAGCGGG |
| Suggested primer 2: | GGGAGGGTGGGTTGTGTATG |