Insertion junction: CLIP2.067275_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.067275
Chromosome:plastome
Location:20437
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802271 ChreCp009,2717052,chlL light-independent protochlorophyllide reductase iron-sulfer ATP-binding protein; (1 of 1) K04037 - light-independent protochlorophyllide reductase subunit L (chlL) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAATCAGCGTAGTTTAATGGAGCAGCAAAGCCACCACAAACAACATCACC
Internal bar code:AAGGTACATCCTCATTGATATA

Confirmation - LEAP-Seq

LEAP-Seq distance:552
LEAP-Seq percent confirming:4.87805
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:39
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCCTTCCCCTTCCCCTT
Suggested primer 2:GAAGCAGCCAACAAATGCCA