| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.067296 |
| Chromosome: | chromosome 13 |
| Location: | 2681772 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g581500 | OPR41 | OctotricoPeptide Repeat protein 41 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTGCTGCAGCATGTGCATAGTGTAGGCGCTCTGCAGCAGCGCCACGC |
| Internal bar code: | GTTATATTGTTGGGTGTCTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 719 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCTTACGCGCTGCTAACA |
| Suggested primer 2: | CGTGGTAATGTGTGGCTCCT |