| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.067309 |
| Chromosome: | chromosome 10 |
| Location: | 3795493 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g445650 | SMC3 | (1 of 1) K06669 - structural maintenance of chromosome 3 (chondroitin sulfate proteoglycan 6) (SMC3, CSPG6); Structural Maintenance of Chromosomes protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCTGCACTAGGACGCTCGCCACTGGTAGCGAATTAGCTGCGACCTCAG |
| Internal bar code: | AACTTATCGGCCGCTCAAGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 628 |
| LEAP-Seq percent confirming: | 53.3333 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCTGCCCCTGAAACTAAG |
| Suggested primer 2: | TTCTTTTTGTCGGCCTTGCG |