| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.067316 |
| Chromosome: | chromosome 10 |
| Location: | 738730 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422550 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | 5'UTR | |
| Cre10.g422600 | NUO6 | NADH:ubiquinone oxidoreductase 51 kDa subunit; (1 of 1) K03942 - NADH dehydrogenase (ubiquinone) flavoprotein 1 (NDUFV1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTCATCGGCTTTGACCGTTCATGGACTTCTGCCTAAGCCTTGAGCCTA |
| Internal bar code: | GGCACGGCTAGCGAGAATGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3868 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGCTGTACTCTGTGTGCT |
| Suggested primer 2: | CAGACGTGGACTCGGTTTCA |