| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.067326 |
| Chromosome: | chromosome 6 |
| Location: | 8733064 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g310601 | TNP19 | putative transposase; (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGAGTCAGGTTCAGGGTTCAGTAGACGTGGACGCTGAGTGCGGCTGAGT |
| Internal bar code: | GATGAAAGGTTTGCAATACACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1243 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTGCGGTTTGCTGTACGT |
| Suggested primer 2: | AAGTGCGGATTTCCATCGGT |