Insertion junction: CLIP2.067384_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.067384
Chromosome:chromosome 4
Location:1359350
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre04.g214502 UGE,DIV113,UGE1,EZY4 (1 of 1) 5.1.3.2//5.1.3.5 - UDP-glucose 4-epimerase / Uridine diphospho-galactose-4-epimerase // UDP-arabinose 4-epimerase; UDP-D-glucose/UDP-D-galactose 4-epimerase 5 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCATACACAAACACGGCACGGCGCGCACATACACACACACGCTCGCTG
Internal bar code:TATTAGTGGTGGTAAAACTGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:150
LEAP-Seq percent confirming:7.69231
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CCCACAAGTGCAAGAACGTG
Suggested primer 2:CCCCAGCTTCCATTTCGAGT