Insertion junction: CLIP2.067390_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.067390
Chromosome:chromosome 2
Location:7250076
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g391134 (1 of 16) IPR003903 - Ubiquitin interacting motif 3'UTR
Cre09.g391171 (1 of 4) 6.3.5.4 - Asparagine synthase (glutamine-hydrolyzing) / Glutamine-dependent asparagine synthetase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAGGCACTGGATACGAAACACGGTTTCAACGCTTACGAACACAATACAC
Internal bar code:GGACATCGTATCAGATTCGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2062
LEAP-Seq percent confirming:98.4848
LEAP-Seq n confirming:65
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:66

Suggested primers for confirmation by PCR

Suggested primer 1:TTTCGGCCGCTTGAGTTTTG
Suggested primer 2:TTGTCATGTTCGGTGAGGCA