| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.067402 |
| Chromosome: | chromosome 6 |
| Location: | 7947449 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g304300 | POA6 | 20S proteasome alpha subunit F; (1 of 1) K02725 - 20S proteasome subunit alpha 6 (PSMA1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCCTCAAGGATGCGATGGAATAGGGGCATAGTCCCGTCGTGTGTAAC |
| Internal bar code: | GTTGTACACGGCGGCGCAGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1429 |
| LEAP-Seq percent confirming: | 97.8723 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCTCTTCGCCTGTATCGG |
| Suggested primer 2: | ACAGTATGCAAAGCGTCCGA |