| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.067406 |
| Chromosome: | chromosome 11 |
| Location: | 1474689 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g469350 | TTL12 | (1 of 14) PF03133 - Tubulin-tyrosine ligase family (TTL); Putative tubulin tyrosine ligase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTACATCGGCTGGCACGTCAGCACCTGTCCACGCGCCCGCAAAAGACT |
| Internal bar code: | CACCTCGCGTGCAGTTCGGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3312 |
| LEAP-Seq percent confirming: | 84.2105 |
| LEAP-Seq n confirming: | 128 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 152 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTGCTCACCGGGGTACTT |
| Suggested primer 2: | CTCGTGTCATGCAGCGATTG |