| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.067453 |
| Chromosome: | chromosome 16 |
| Location: | 3602638 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g672041 | (1 of 1) 2.1.1.228 - tRNA (guanine(37)-N(1))-methyltransferase / tRNA-(N(1)G37) methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGACTTTCGGGACACCCAGTGTGATCCGGCAGAAATATGTCCAACCAGA |
| Internal bar code: | ACAGAGTCCATAAGACCATGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3061 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCTGTACCAATGCTTCCT |
| Suggested primer 2: | CAGCGTATGTGATTGCTGCC |