Insertion junction: CLIP2.067454_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.067454
Chromosome:chromosome 16
Location:2762003
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g662200 CYA14 Putative adenylate cyclase; (1 of 1) 3.6.1.25//3.6.1.3 - Triphosphatase // Adenosinetriphosphatase / Triphosphatase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCTACACCCGCCGCGCAACTTCCGCAACACACCACCCACCTCCGTACC
Internal bar code:ATAACTAATGTTTTAGGAACTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2587
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTGTCAACAAACCTGCGG
Suggested primer 2:GTACCGGCAGATCACCTACG