| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.067464 |
| Chromosome: | chromosome 6 |
| Location: | 5653297 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g287750 | PYR8 | Dihydroorotase dehydrogenase; (1 of 1) 1.3.1.14 - Dihydroorotate dehydrogenase (NAD(+)) / Orotate reductase (NADH) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCAGCAGCCGCTCAACGCACGTGCGCCCTACCCGCCCGGCGTGCCGC |
| Internal bar code: | GATTGTCCTGACTATTACTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 435 |
| LEAP-Seq percent confirming: | 16.6667 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTCAAACCCTAGCCCTCC |
| Suggested primer 2: | ATATACGTGCTTGAGGCCGG |