Insertion junction: CLIP2.067465_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.067465
Chromosome:chromosome 12
Location:2533507
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g507001 3'UTR
Cre12.g507002 (1 of 38) IPR017448 - SRCR-like domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTAGGTTGGCGTGCGTGCGTGTTGGGGTCTAGGGCGGGCGCCTACACG
Internal bar code:TCAACGGTTCACATTGGGCCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2265
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:AAATTTTGCTCGTCGCCAGC
Suggested primer 2:TTTTTGTCAACGCAGGCAGG