Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.067474 |
Chromosome: | chromosome 13 |
Location: | 673647 |
Confidence (%): | 63 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g566300 | RRM12,RSMD | (1 of 1) 2.1.1.171 - 16S rRNA (guanine(966)-N(2))-methyltransferase / M(2)G966 methyltransferase; Putative rRNA (guanine-N2-)-methyltransferase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACACAACCCTGATCCACACAACCCCACACCCTGCAGTCGCCACCAGCGC |
Internal bar code: | AAGTATATTCTGGAGCCCGCCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 793 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 19 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTTCCACGCCCTGTTTTGTG |
Suggested primer 2: | GGGTCGTTTTGTTGCAGGTC |