| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.067477 |
| Chromosome: | chromosome 6 |
| Location: | 8311581 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g307200 | (1 of 1) PTHR10358 - ENDOSULFINE | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAACTTCTCTTGCGGCATCTCCGGACTCGTCGGCACTGCCGGTCCAGTG |
| Internal bar code: | CATAAGACTTCGACCTCGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4082 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGATGGCAATACGCATGCG |
| Suggested primer 2: | CCCCACAAAAGCAGCTTGTC |