| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.067499 |
| Chromosome: | chromosome 12 |
| Location: | 5588949 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g531100 | PBA2 | 20S proteasome beta subunit A2; (1 of 2) K02738 - 20S proteasome subunit beta 1 (PSMB6) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACTACCGGTACTGAAGACGCGGATGCCTCATCAAGATGCATGGCGGCT |
| Internal bar code: | CTGATACTAAGTGGTTAAATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1303 |
| LEAP-Seq percent confirming: | 27.5 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGTGGTGTCTGCGTGTA |
| Suggested primer 2: | CTGCTGACACATTGTTGGCC |