| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.067500 |
| Chromosome: | chromosome 10 |
| Location: | 6609276 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g465850 | (1 of 1) K12572 - PAB-dependent poly(A)-specific ribonuclease subunit 3 (PAN3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGAGGTGCCTCCTCCTGTGGCAGTGGGGCGCGACCGATCCATACAGTG |
| Internal bar code: | GATGTACTATTCAGATTGCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2636 |
| LEAP-Seq percent confirming: | 95.0 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCACTCTACCCTCCCGAC |
| Suggested primer 2: | GAAAGAGGAAGGCACGGGAA |