| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.067521 |
| Chromosome: | chromosome 6 |
| Location: | 3094324 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g275300 | (1 of 3) PF05670 - Domain of unknown function (DUF814) (DUF814) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGAACTTGCAGCGGCGGCGCCTGGAAAACGGCTGCGCTAACGCGGAGG |
| Internal bar code: | CGCCTTTGTATTCACGTAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 524 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCCTAGTCAAGCAACCCC |
| Suggested primer 2: | GATGTCATTCGTCGTGCGTG |