| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.067523 |
| Chromosome: | chromosome 12 |
| Location: | 2204255 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g509000 | MAPK3 | Mitogen-Activated Protein Kinase 3; (1 of 3) K04371 - mitogen-activated protein kinase 1/3 (MAPK1_3) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCACACTCAAACACTTGCCACATTTTGCCGCACACAAATCAATATGTC |
| Internal bar code: | TTAGCACGCAGGTGAGGTCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5043 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGATAGGCAGGGTGAGA |
| Suggested primer 2: | CTCGAGCGCCTCTCATTGAT |