Insertion junction: CLIP2.067558_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.067558
Chromosome:chromosome 6
Location:2082928
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g265950 DHC3 Axonemal Inner Arm Dynein Heavy Chain 3, pseudogene; (1 of 2) PTHR10676:SF136 - DYNEIN HEAVY CHAIN 6, AXONEMAL intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAACGGAGACTCGTGGCTGAGCGCAATGCCGTCACACATCACGCGAGCAT
Internal bar code:CTTGTTAGTTTGGCTTCTTCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:4125
LEAP-Seq percent confirming:88.4615
LEAP-Seq n confirming:92
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:104

Suggested primers for confirmation by PCR

Suggested primer 1:GGACTTCAGGTTCATGGGCA
Suggested primer 2:CGTATGAGCACCGTCTCCTC