| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.067566 |
| Chromosome: | chromosome 8 |
| Location: | 1486041 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g364800 | (1 of 1) 6.3.5.3 - Phosphoribosylformylglycinamidine synthase / Phosphoribosylformylglycinamidine synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGGGGGGGGGGGCGAAGCGAGTGCGGGGGCGCAGCGGTGTGGTTGTGG |
| Internal bar code: | GGGCGCTTTAAAAGGATATCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 598 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTAAGGTGGTGGTGGTGG |
| Suggested primer 2: | CATCCCCTCCCCCTTCCTTA |