| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.067579 |
| Chromosome: | chromosome 11 |
| Location: | 3104481 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g475200 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTAGCTAAAACGTCATCGCATTGTGTAGTAAGGGAGGGGGCTGGAAAGT |
| Internal bar code: | GATCGGTATCTTAGCACTGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3652 |
| LEAP-Seq percent confirming: | 95.4545 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCTAGGTTTTGCCTCGGT |
| Suggested primer 2: | CTCGTAGCTCAGAGGAAGCG |