| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.067580 |
| Chromosome: | chromosome 17 |
| Location: | 750929 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g701100 | CPK8 | (1 of 1) 2.7.1.83 - Pseudouridine kinase; putative carbohydrate kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGCATGTGCAGTGCGGCACACTCTATGCAGGGGGCGCTTCACGCCCCC |
| Internal bar code: | GTTAAGTACCATGTGTGTTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2203 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGCTGGGCTTGATCTCT |
| Suggested primer 2: | GGGTTGGGATTCTGAGGGTG |