| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.067592 |
| Chromosome: | chromosome 9 |
| Location: | 3244057 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394806 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGTGTCGAGGGGATGGCTGGTGGGTGTTATGTCATCTTGGCGTCAAAT |
| Internal bar code: | TTTCAGTAGTGTGGGCCCAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1701 |
| LEAP-Seq percent confirming: | 95.7143 |
| LEAP-Seq n confirming: | 67 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCGTTGCAGGTCACAATT |
| Suggested primer 2: | TGTCGTCTGTAAAGCGGGAC |