| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.067596 |
| Chromosome: | chromosome 14 |
| Location: | 3799296 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g632176 | (1 of 1) PTHR10641:SF660 - DNA-BINDING PROTEIN REB1-RELATED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGAGACACACACACAGCGCGCGGCTGTAGGCGCGACCTGGGGTGTCTCC |
| Internal bar code: | ATCCGCACCTCGACTTCCTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4774 |
| LEAP-Seq percent confirming: | 86.6667 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTGACGTTTGAAGCACG |
| Suggested primer 2: | TGATGGTGGTGATGGTGGTG |