| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.067597 |
| Chromosome: | chromosome 13 |
| Location: | 4849655 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g605050 | (1 of 1) K15075 - DNA repair/transcription protein MET18/MMS19 (MET18, MMS19) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTACCGTCTACTCATTCCGTAGCGTATCATGAACGTAGCCCCCATTCC |
| Internal bar code: | GGAGCTTGCCACCCTGGGGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 59 |
| LEAP-Seq percent confirming: | 21.4286 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAACCCATGCTCCCTCAAC |
| Suggested primer 2: | CCGTACAGCATACCTCCGTC |