| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.067600 |
| Chromosome: | chromosome 12 |
| Location: | 9414535 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g541250 | NAR1E | Putative nitrite transporter; (1 of 7) PTHR30520//PTHR30520:SF0 - FORMATE TRANSPORTER-RELATED // FORMATE TRANSPORTER 1-RELATED | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCTGAGCCATTGAGCCGACCGCGCTGACCAACGTCAATCTTCGCGCGC |
| Internal bar code: | TTATGAGCTATGCAGGTTCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 194 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAATTGCGGCACAGGTTT |
| Suggested primer 2: | CCGTCCTCGAGTATGCTGAC |