| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.067601 |
| Chromosome: | chromosome 16 |
| Location: | 4752408 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687950 | ETFA1,ETF1,ETFA | Electron transfer flavoprotein alpha subunit; (1 of 1) 1.3.1.95 - Acryloyl-CoA reductase (NADH) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAATGCTTCAGGCGAGGGGGCGGGCCGCCGTTAACGCACGGTATCCTCGC |
| Internal bar code: | TGGGGTAGTGGTGGAAAGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3027 |
| LEAP-Seq percent confirming: | 59.5745 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 94 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGCTTTAGCCTCATCGC |
| Suggested primer 2: | GCACTTGGCACGTTACGTTT |